human tumor derived cell lines  (ATCC)

Journal: STAR Protocols

Article Title: Protocol for dual metabolomics and proteomics using nanoflow liquid chromatography-tandem mass spectrometry

doi: 10.1016/j.xpro.2025.103745

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Article Snippet: Timing: 2.5 days The present protocol uses the human breast cancer-derived cell line MDA-MB-231 obtained from the American Type Culture Collection (ATCC) as the model organism.

Techniques: Recombinant, Modification, Liquid Chromatography with Mass Spectroscopy, Sequencing, Bicinchoninic Acid Protein Assay, Mass Spectrometry, Software

Journal: Antioxidants

Article Title: Myrobalan Fruit Extracts Modulate Immunobiochemical Pathways In Vitro

doi: 10.3390/antiox14030350

Figure Lengend Snippet: Effects of BB3 and the fruit extracts on cell viability. HepG2 cells were treated with vehicle (EtOH) or BB3 or myrobalan extracts (12.5–200 μg/mL) for 24 ( A ), 48 ( B ), and 72 h ( C ). Mean values ± S.E.M. of four independent experiments run in duplicates (* p < 0.05 and ** p < 0.005; compared to untreated cells) are shown.

Article Snippet: The human liver cancer-derived cell line HepG2 (RRID:CVCL_0027; DSMZ, Germany) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco—Thermo Fisher Scientific, Vienna, Austria) supplemented with 10% ( v / v ) heat-inactivated FCS.

Techniques:

Journal: Antioxidants

Article Title: Myrobalan Fruit Extracts Modulate Immunobiochemical Pathways In Vitro

doi: 10.3390/antiox14030350

Figure Lengend Snippet: Measurement of intracellular ROS. Inhibition of peroxyl-radical (AAPH)-induced formation of ROS in HepG2 cells pretreated with increasing concentrations of BB3 or the myrobalan extracts (12.5–200 μg/mL). The mean percentages of DCF fluorescence, as a measure of ROS formation, are shown in relation to the AAPH-treated EtOH vehicle control (set to 100%). Mean values ± S.E.M. of three independent experiments run in quadruplicates (** p < 0.01; compared to AAPH-treated cells) are shown.

Article Snippet: The human liver cancer-derived cell line HepG2 (RRID:CVCL_0027; DSMZ, Germany) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco—Thermo Fisher Scientific, Vienna, Austria) supplemented with 10% ( v / v ) heat-inactivated FCS.

Techniques: Inhibition, Fluorescence, Control

Journal: Antioxidants

Article Title: Myrobalan Fruit Extracts Modulate Immunobiochemical Pathways In Vitro

doi: 10.3390/antiox14030350

Figure Lengend Snippet: Influence of plant extracts on ß-lactamase activity, which was taken as a measure for ARE-dependent gene expression, in CellSensor ® ARE-bla HepG2 cells (expressed as n-fold to the corresponding control solvent). Shown are the mean values ± S.E.M. of three independent experiments with four parallels per concentration. (* p < 0.05 and ** p < 0.01; compared to vehicle-treated control cells).

Article Snippet: The human liver cancer-derived cell line HepG2 (RRID:CVCL_0027; DSMZ, Germany) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco—Thermo Fisher Scientific, Vienna, Austria) supplemented with 10% ( v / v ) heat-inactivated FCS.

Techniques: Activity Assay, Gene Expression, Control, Solvent, Concentration Assay

Journal: ACS Omega

Article Title: Novel C-2 Aromatic Heterocycle-Substituted Triterpenoids Inhibit Hedgehog Signaling in GLI1 Overexpression Cancer Cells

doi: 10.1021/acsomega.4c11479

Figure Lengend Snippet: Effect of compounds 11a and 11b on the protein expression of the Gli1 transcription factor and its transcriptional targets in A549 (A) and DU145 (B) cells following 24 h of incubation. An antibody against GAPDH was used as an internal loading control.

Article Snippet: The human prostate tumor-derived cell line DU145 was obtained from ATCC (American Type Culture Collection) and cultured in RPMI 1640 (Roswell Park Memorial Institute) medium supplemented with 10% (v/v) fetal bovine serum (FBS).

Techniques: Expressing, Incubation, Control

Journal: ACS Omega

Article Title: Novel C-2 Aromatic Heterocycle-Substituted Triterpenoids Inhibit Hedgehog Signaling in GLI1 Overexpression Cancer Cells

doi: 10.1021/acsomega.4c11479

Figure Lengend Snippet: Effect of compounds 11a and 11b on the protein expression of the Gli1 transcription factor and its transcriptional targets in A549 (A) and DU145 (B) cells following 24 h of incubation. An antibody against GAPDH was used as an internal loading control.

Article Snippet: The human lung tumor-derived cell line A549 was obtained from ATCC and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS.

Techniques: Expressing, Incubation, Control